High-Performance Liquid Chromatography Tandem Mass Spectrometry (HPLC-MS/MS) is a powerful analytical technique combining the physical separation capabilities of HPLC with the mass analysis capabilities of mass spectrometry (MS). Liquid chromatography separates mixtures with multiple components and the mass spectrometry provides spectral information that helps to identify or confirmed the supsected identity of each separated component.

HPLC-MS/MS is widely used for both quantitative and qualitative analysis of compounds. In quantitative analysis, the technique offers high sensitivity and selectivity, allowing accurate determination of trace-level analytes in complex matrices. In qualitative analysis, HPLC-MS/MS provides structural information about compounds, including their fragmentation patterns, which can be used for identification and characterization.

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